Homemade Yogurt with Live Active Cultures
A recipe from From Scratch.
From ScratchYogurtLiveCulturesFromScratchFermentedProbioticHomemadeBreakfastBase

Nutrition (per serving)
150Calories
10 gProtein
12 gCarbs
8 gFat
12 gSugar
120 mgSalt
Ingredients
- 1 liter Whole milk (or 2% / low-fat)
- 2–3 tbsp (must contain live cultures) Plain yogurt with live active cultures (store-bought starter)
Method
- Heat milk in a heavy-bottomed pot over medium heat to 82–85 °C (180–185 °F) — stir occasionally to prevent scorching. Use a thermometer.
- Hold at this temperature for 5–10 minutes (kills unwanted bacteria and denatures proteins for thicker yogurt).
- Remove from heat → let cool to 43–46 °C (110–115 °F) — stir occasionally to speed cooling.
- In a small bowl, whisk store-bought yogurt starter until smooth → temper by slowly adding ½ cup warm milk while whisking.
- Pour tempered starter back into the pot of cooled milk → stir gently but thoroughly for 1 minute.
- Pour mixture into clean glass jars or a large container → cover loosely.
- Incubate: Place jars in oven with light on (or use yogurt maker, Instant Pot yogurt setting, or wrap in towels near warm spot) at 40–43 °C (104–110 °F) for 6–12 hours (longer = tangier & thicker). Do not disturb during fermentation.
- Check after 6 hours: yogurt should be set like thick custard → if not, continue incubating up to 12 hours.
- Once set, refrigerate 4–6 hours to stop fermentation and firm up.
- Stir before serving — store in fridge up to 2 weeks. Save 2–3 tbsp as starter for next batch.
- Makes 1 liter — creamy, tangy homemade yogurt with live active cultures.
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